![]() After you have reached a satisfactory doublet, freeze all variables and add another surface to the end of your system. Then, if you wish to change the glasses in your system, run a hammer optimization. ![]() Set some preliminary radii before running a local optimization on the lens. Apply the default merit function settings, optimizing on minimum RMS spot radius, as shown below. Using the glass substitution template option in the material column is optional. Vary only the radii of the lens surfaces. The constraints for the example system’s laser portion are as follows: Wavelengthįirst create the surfaces needed for focusing optics, selecting any glass(es) for the materials column. The design for the laser focusing system will first be done in Sequential Mode. Note: While this microscope is not designed to be a scanning confocal fluorescent microscope, a group of laser collimating optics is included in the example design to serve as a reference template for where to modify the design into a scanning confocal microscope. The placement of these pinholes at conjugate points is what makes this design a confocal microscope. The second pinhole is placed after the tube lens in front of a photo-detector. The first pinhole is placed between the laser focusing and collimating optics. The thinner red beam is included to illustrate the purpose of the second pinhole. The thicker red beam represents the in-focus fluorescence captured by the detector. The purple beam represents the propagation of the laser source. These optics are configured in the following orientation: System overviewĪ confocal fluorescent microscope's optical system consists of a laser illumination source, a focusing lens, a collimating lens, a microscope objective, a tube lens, and a detector. ![]() This article will provide a walk-through of the design to show how to acurrately model confocal microscopes in OpticStudio. The microscope objective used in this example is the "Microscope Objective 60x" available in Zemax Design Templates (or file example K_007 previously in Zebase), which is available to all editions of Subscription OpticStudio 20.2 or above. To generate such high quality results, the microscopes are designed in two major parts: from the laser source to the microscope objective, and from the microscope objective to the detector. It also discusses how to build a merit funciton for optimization and how to use the Convert to NSC Group tool to convert system from Sequential Mode to Non-Sequential Mode.Ĭonfocal fluorescent microscopy is a means of obtaining high resolution 3D images of a sample and is especially useful in the life sciences and in the semiconductor industry. This article provides a walk-through of the design of the confocal microscope. The system is designed in two major parts: from the laser source to the microscope objective, and from the microscope objective to the tube lens and detector. This article shows how to design a confocal fluorescent microscope in OpticStudio using a combination of the Sequential and Non-Sequential Modes. ![]()
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